Micropropagation and experimental field cultivation of Pulsatilla turczaninovii Kryl. et Serg. (Ranunculaceae)

Abstract Pulsatilla turczaninovii is an important medicinal plant, valued for high ornamental value of melliferous flowers. We assessed the efficiency reproduction under in vitro conditions and ex situ growth capacity this representative world flora. The seed germination percentage was assessed, followed by determination micropropagation rate rooting efficiency. Then, possibility plant development three consecutive growing seasons assessed. approximately 55%. highest multiplication coefficient, amounting to 5.17, obtained on modified MS medium supplemented with 2.5 mg L −1 2iP 1.0 IAA. Our study provided unique insight biochemical background root regeneration P. . In comparison standard auxin-supplemented medium, treatment level ethylene precursor ACC elevated about 20%. total content soluble sugars proved be biomarker rhizogenesis studied species. Their concentration positively correlated efficiency, while a phenolic length regenerated roots, their number per rosette. cultivation acclimatized material successfully carried out evaluated over subsequent years. third year cultivation, plants entered stage generative most them bloomed profusely.